Pinealon Reconstitution: How Much Bacteriostatic Water

A 10 mg vial of Pinealon reconstituted with 2 mL of bacteriostatic water gives a concentration of 5 mg/mL; with 1 mL it gives 10 mg/mL. A 20 mg vial with 2 mL gives 10 mg/mL. The calculation depends only on the quantity in the vial and the volume of water added, so the figures below apply to any Pinealon vial of these sizes.

Peptide reconstitution calculator

Set to this vial below. Change the vial size, water volume, amount or syringe and it recalculates the concentration and the syringe units. For laboratory research use only.

Desired dose

0.5 mg 1 mg 1.5 mg 2 mg 2.5 mg 3 mg 3.5 mg 4 mg 4.5 mg 5 mg Custom… Milligrams (mg) Micrograms (mcg)

1 mg = 1000 mcg

Research peptide in vial (mg) 5 mg 10 mg 20 mg 30 mg Custom… As printed on the vial

Bacteriostatic water added (ml) 1 mL 2 mL 3 mL Custom… Total volume you put in the vial

Syringe size 0.3 ml — 30 units 0.5 ml — 50 units 1.0 ml — 100 units Custom…

Draw to 5.0units

Concentration—

Volume per dose—

Doses per vial—

All products are sold strictly for laboratory research use only and are not for human or veterinary consumption. This calculator is provided as a general reference tool.

How much bacteriostatic water for 10 mg Pinealon?

A 10 mg Pinealon vial is commonly reconstituted with 2 mL of bacteriostatic water, which gives 5 mg/mL; 1 mL gives 10 mg/mL, 3 mL gives 3.33 mg/mL and 5 mL gives 2 mg/mL. The table shows how much Pinealon each 0.1 mL of solution contains at each volume. Adding more water does not change the 10 mg in the vial; it only lowers the concentration.

Water added	Concentration	Amount in 0.1 mL (10 units on a U-100 syringe)
1 mL	10 mg/mL	1 mg
2 mL	5 mg/mL	0.5 mg
3 mL	3.33 mg/mL	0.33 mg
5 mL	2 mg/mL	0.2 mg

For any other water volume, enter it in the peptide calculator on this page and it works out the concentration.

How much bacteriostatic water for 20 mg Pinealon?

A 20 mg Pinealon vial is commonly reconstituted with 2 mL of bacteriostatic water, which gives 10 mg/mL; 1 mL gives 20 mg/mL, 4 mL gives 5 mg/mL and 5 mL gives 4 mg/mL. The table shows how much Pinealon each 0.1 mL of solution contains at each volume. Adding more water does not change the 20 mg in the vial; it only lowers the concentration.

Water added	Concentration	Amount in 0.1 mL (10 units on a U-100 syringe)
1 mL	20 mg/mL	2 mg
2 mL	10 mg/mL	1 mg
4 mL	5 mg/mL	0.5 mg
5 mL	4 mg/mL	0.4 mg

For any other water volume, enter it in the peptide calculator on this page and it works out the concentration.

Before choosing a larger volume, check that the vial has headspace for it. One multi-dose vial of bacteriostatic water covers several research vials.

Reading the concentration on a U-100 syringe

A U-100 insulin syringe is graduated in units, and 100 units equal 1 mL. Units are therefore millilitres multiplied by 100: 0.1 mL is 10 units and 0.25 mL is 25 units. A 0.5 mL syringe carries 50 units and a 0.3 mL syringe carries 30 units on the same scale, with finer markings that make small volumes easier to read.

To find the volume that contains a given amount, divide the amount by the concentration. At 5 mg/mL (a 10 mg vial with 2 mL of water), the volume that contains 1 mg is 1 divided by 5, which is 0.2 mL, or 20 units. The table below runs the same calculation for four arbitrary amounts. They illustrate the arithmetic and nothing else.

Amount (illustrative)	Volume at 5 mg/mL	U-100 units
0.25 mg	0.05 mL	5 units
0.5 mg	0.1 mL	10 units
1 mg	0.2 mL	20 units
2 mg	0.4 mL	40 units

At 10 mg/mL, which the same vial gives with 1 mL of water, every volume in the table moves in proportion: a 1 mg draw measures 10 units instead of 20. Volumes below about 0.1 mL are hard to read accurately on a 1 mL syringe. Either move to a 0.3 mL or 0.5 mL syringe, or reconstitute with more water so that the same mass occupies a larger, more legible volume.

What Pinealon is

Pinealon is a synthetic tripeptide, glutamic acid, aspartic acid and arginine (Glu-Asp-Arg, often written EDR), one of the short regulatory peptides studied by Vladimir Khavinson’s group in Saint Petersburg. Its research centres on gene expression in nerve cells and on markers of ageing.

• In a study of people aged 41 to 83 with chronic polymorbidity and organic brain syndrome in remission, Pinealon and the related tripeptide Vesugen showed an anabolic effect, improved central nervous system activity and slowed the rate of ageing measured by biological age indicators (Meshchaninov et al., 2015).
• A 2020 review describes the EDR peptide entering cells and binding histone proteins, changing the synthesis of proteins involved in neuronal function, including the antioxidant enzymes SOD2 and GPX1, and reports improved memory in elderly patients (Khavinson et al., 2020).
• In ageing cultures of brain cortex cells, Glu-Asp-Arg stimulated serotonin expression, and molecular docking matched it to a sequence in the gene for tryptophan hydroxylase, the enzyme behind serotonin synthesis (Khavinson et al., 2014).

The Pinealon vial

Pinealon is commonly supplied in research catalogues as a lyophilised powder in 10 mg and 20 mg vials. Vistara Labs stocks Pinealon as a 10 mg lyophilised vial. The calculator on this page works for any vial. Vistara Labs supplies Cerebrolysin, a 60 mg vial that is also prepared with bacteriostatic water.

Reconstitution technique

Let the vial reach room temperature, swab both stoppers, and run the measured water slowly down the inside wall of the vial rather than onto the powder. Swirl or tilt gently until the cake dissolves, never shake, and give any remaining powder a few minutes to settle into solution. Label the vial with the date and the concentration, then keep it refrigerated at 2 to 8 °C and protected from light. The full method, with the reason behind each step, is in how to reconstitute peptides with bacteriostatic water.

Why the solution should look clear

Properly reconstituted Pinealon forms a clear, colourless solution. Cloudiness, strands or a gel that will not disperse usually mean the water went in too fast or the vial was shaken; haze that appears later points to warm or bright storage. The causes are covered in why peptides gel or go cloudy.

Bacteriostatic water, not sterile water

Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. The difference is explained in bacteriostatic water explained.

Frequently asked questions

How much bac water for a 10 mg Pinealon vial?

A 10 mg Pinealon vial is commonly reconstituted with 2 mL of bacteriostatic water, which gives 5 mg/mL; 1 mL gives 10 mg/mL, 3 mL gives 3.33 mg/mL and 5 mL gives 2 mg/mL. Adding more water does not change the 10 mg in the vial; it only lowers the concentration.

How much bac water for a 20 mg Pinealon vial?

A 20 mg Pinealon vial is commonly reconstituted with 2 mL of bacteriostatic water, which gives 10 mg/mL; 1 mL gives 20 mg/mL, 4 mL gives 5 mg/mL and 5 mL gives 4 mg/mL. Adding more water does not change the 20 mg in the vial; it only lowers the concentration.

How many 10 mg vials does one vial of bacteriostatic water cover?

One multi-dose vial of bacteriostatic water covers several research vials. Check that the peptide vial has headspace for the volume before choosing a larger one.

How many units on a U-100 syringe contain 1 mg at 5 mg/mL?

At 5 mg/mL (a 10 mg vial with 2 mL of water), the volume that contains 1 mg is 1 divided by 5, which is 0.2 mL, or 20 units. Units are millilitres multiplied by 100, so 0.1 mL is 10 units. The amounts on this page illustrate the arithmetic only.

What should reconstituted Pinealon look like?

Properly reconstituted Pinealon forms a clear, colourless solution. Cloudiness, strands or a gel that will not disperse usually mean the water went in too fast or the vial was shaken; haze that appears later points to warm or bright storage. More on the causes in why peptides gel or go cloudy.

Can I use sterile water instead of bacteriostatic water?

Only for a vial that will be accessed once. Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. Details in bacteriostatic water explained.

How should a reconstituted Pinealon vial be stored?

Refrigerated at 2 to 8 °C, protected from light and labelled with the date and the concentration. Unopened lyophilised vials are kept cold and dark until they are needed. The full storage guide is how to store research peptides.

Does Vistara Labs sell Pinealon?

Vistara Labs stocks Pinealon as a 10 mg lyophilised vial. The calculator on this page works for any vial.

Work it out for a different vial or concentration

Our peptide calculator opens pre-set for a 10 mg vial with 2 mL of water and marks the 1 mg draw of 20 units on a syringe diagram. Change the vial size, water volume, amount or syringe and it recalculates. For a 20 mg vial, set the vial size to 20.

Products: bacteriostatic water.

Related reading. How to reconstitute peptides, how to store research peptides and bacteriostatic water explained.

Sources: Meshchaninov VN et al. Effect of synthetic peptides on aging of patients with chronic polymorbidity and organic brain syndrome of the central nervous system in remission (in Russian). Adv Gerontol. 2015. PMID 26390612. Khavinson V et al. EDR Peptide: Possible Mechanism of Gene Expression and Protein Synthesis Regulation Involved in the Pathogenesis of Alzheimer’s Disease. Molecules. 2020. PMID 33396470. Khavinson VKh et al. Short peptides stimulate serotonin expression in cells of brain cortex. Bull Exp Biol Med. 2014. PMID 24909721.