How Much Bacteriostatic Water for a 5-Amino-1MQ 50 mg Vial?
A 50 mg vial of 5-Amino-1MQ reconstituted with 2 mL of bacteriostatic water gives a concentration of 25 mg/mL; with 1 mL it gives 50 mg/mL. The calculation depends only on the quantity in the vial and the volume of water added, so the figures below apply to any 50 mg vial.
Voir le produit →There is no single correct volume. Adding more water does not change how much is in the vial. It spreads the same quantity through a larger volume, which changes how many syringe units correspond to a given amount. The tables on this page use volumes that produce round numbers on a U-100 syringe; other volumes work identically as long as the resulting concentration is written on the vial.
Concentration by water volume
Concentration is the quantity in the vial divided by the water volume. A 50 mg vial holds 50 mg whether it receives 1 mL or 5 mL; only the mg/mL figure moves. The right-hand column converts each concentration into the number of U-100 units that contain 5 mg.
| Vial | Bacteriostatic water added | Concentration | Units containing 5 mg |
|---|---|---|---|
| 50 mg 5-Amino-1MQ | 1 mL | 50 mg/mL | 10 units |
| 50 mg | 2 mL | 25 mg/mL | 20 units |
| 50 mg | 5 mL | 10 mg/mL | 50 units |
Before choosing a larger volume, check that the vial has headspace for it. A 10 mL vial of bacteriostatic water reconstitutes five 50 mg vials at 2 mL each.
Reading the concentration on a U-100 syringe
A U-100 insulin syringe is graduated in units, and 100 units equal 1 mL. Units are therefore millilitres multiplied by 100: 0.1 mL is 10 units and 0.25 mL is 25 units. A 0.5 mL syringe carries 50 units and a 0.3 mL syringe carries 30 units on the same scale, with finer markings that make small volumes easier to read.
To find the volume that contains a given amount, divide the amount by the concentration. At 25 mg/mL (a 50 mg vial with 2 mL of water), the volume that contains 5 mg is 5 divided by 25, which is 0.2 mL, or 20 units. The table below runs the same calculation for four arbitrary amounts. They illustrate the arithmetic and nothing else.
| Amount (illustrative) | Volume at 25 mg/mL | U-100 units |
|---|---|---|
| 2.5 mg | 0.1 mL | 10 units |
| 5 mg | 0.2 mL | 20 units |
| 10 mg | 0.4 mL | 40 units |
| 20 mg | 0.8 mL | 80 units |
At 50 mg/mL, which the same vial gives with 1 mL of water, every volume in the table moves in proportion: a 10 mg draw measures 20 units instead of 40. Volumes below about 0.1 mL are hard to read accurately on a 1 mL syringe. Either move to a 0.3 mL or 0.5 mL syringe, or reconstitute with more water so that the same mass occupies a larger, more legible volume.
5-Amino-1MQ is supplied by Vistara Labs as a lyophilised material for research use. The quantities on this page illustrate the arithmetic of dilution and measurement only.
The 5-Amino-1MQ vial
5-Amino-1MQ is a small molecule, an NNMT inhibitor, not a peptide, supplied as a 50 mg vial of deep red powder. The red is the molecule itself, not a dye, and it carries straight into the solution. Fifty milligrams is five times a standard peptide vial, so the table starts higher: 2 mL gives 25 mg/mL. Certificates of analysis for the catalogue are published on the Résultats de laboratoire page.
Because 5-Amino-1MQ is a small molecule rather than a peptide, 50 mg of it contains many times more individual molecules than 50 mg of a typical peptide. It works by inhibiting NNMT, an enzyme inside fat cells, and its research to date is laboratory work in cells and mice; no human trials have been published. The detail is in 5-Amino-1MQ: what the NNMT research actually covers, and the buying questions in buying 5-Amino-1MQ in Canada.
Reconstitution technique
Let the vial reach room temperature, swab both stoppers, and run the measured water slowly down the inside wall of the vial rather than onto the powder. Swirl or tilt gently until the cake dissolves, never shake, and give any remaining powder a few minutes to settle into solution. Label the vial with the date and the concentration, then keep it refrigerated at 2 to 8 °C and protected from light. The full method, with the reason behind each step, is in l'achat d'eau bactériostatique au Canada.
Why the solution is red
Properly reconstituted 5-Amino-1MQ forms a clear red solution: the colour belongs to the molecule and deepens with concentration. What should not appear is cloudiness or particles that will not dissolve after standing, which usually means the water went in too fast or the vial was shaken. Turbidity that appears after a period of storage can mean the solution has been kept too warm or exposed to light. The causes are covered in more detail in why peptides gel or go cloudy.
Bacteriostatic water, not sterile water
Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. The difference is explained in bacteriostatic water explained.
Questions fréquentes
How much bacteriostatic water do I add to a 50 mg vial of 5-Amino-1MQ?
There is no single correct volume. With 2 mL of bacteriostatic water a 50 mg vial of 5-Amino-1MQ gives 25 mg/mL; with 1 mL it gives 50 mg/mL. Adding more water does not change the quantity in the vial, it spreads it through a larger volume, so write the concentration you chose on the vial.
How many 50 mg vials does a 10 mL vial of bacteriostatic water cover?
A 10 mL vial of bacteriostatic water reconstitutes five 50 mg vials at 2 mL each. Check that the peptide vial has headspace for the volume before choosing a larger one.
How many units on a U-100 syringe contain 5 mg at 25 mg/mL?
At 25 mg/mL (a 50 mg vial with 2 mL of water), the volume that contains 5 mg is 5 divided by 25, which is 0.2 mL, or 20 units. Units are millilitres multiplied by 100, so 0.1 mL is 10 units. The amounts on this page illustrate the arithmetic only.
What should reconstituted 5-Amino-1MQ look like?
Properly reconstituted 5-Amino-1MQ forms a clear red solution: the colour belongs to the molecule and deepens with concentration. What should not appear is cloudiness or particles that will not dissolve after standing, which usually means the water went in too fast or the vial was shaken. Turbidity that appears after a period of storage can mean the solution has been kept too warm or exposed to light. More on the causes in why peptides gel or go cloudy.
Can I use sterile water instead of bacteriostatic water?
Only for a vial that will be accessed once. Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. Details in bacteriostatic water explained.
How should a reconstituted 5-Amino-1MQ vial be stored?
Refrigerated at 2 to 8 °C, protected from light and labelled with the date and the concentration. Unopened lyophilised vials are kept cold and dark until they are needed. The full storage guide is how to store research peptides.
Work it out for a different vial or concentration
Our calculateur de peptides opens pre-set for a 50 mg vial with 2 mL of water and marks the 5 mg draw of 20 units on a syringe diagram. Change the vial size, water volume, amount or syringe and it recalculates.
Products: 5-Amino-1MQ 50 mg et bacteriostatic water 10 mL.
Références
5-Amino-1MQ is a small-molecule NNMT inhibitor rather than a peptide. No completed human clinical trial was identified; the research below is preclinical.
- Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in miceCharacterises 5-Amino-1MQ as a selective, cell-permeable NNMT inhibitor in laboratory studies. No human trial identified.
Les liens ouvrent la fiche de l'étude sur PubMed afin que la source puisse être vérifiée directement. Citer une étude ne signifie pas que ses résultats s'appliquent à un autre usage, produit ou mode d'administration. Usage laboratoire uniquement.
