How Much Bacteriostatic Water for a NAD+ 500 mg Vial?
A 500 mg vial of NAD+ reconstituted with 5 mL of bacteriostatic water gives a concentration of 100 mg/mL; with 2.5 mL it gives 200 mg/mL. The calculation depends only on the quantity in the vial and the volume of water added, so the figures below apply to any 500 mg vial.
Voir le produit →There is no single correct volume. Adding more water does not change how much is in the vial. It spreads the same quantity through a larger volume, which changes how many syringe units correspond to a given amount. The tables on this page use volumes that produce round numbers on a U-100 syringe; other volumes work identically as long as the resulting concentration is written on the vial.
Concentration by water volume
Concentration is the quantity in the vial divided by the water volume. A 500 mg vial holds 500 mg whether it receives 2.5 mL or 10 mL; only the mg/mL figure moves. The right-hand column converts each concentration into the number of U-100 units that contain 50 mg.
| Vial | Bacteriostatic water added | Concentration | Units containing 50 mg |
|---|---|---|---|
| 500 mg NAD+ | 2.5 mL | 200 mg/mL | 25 units |
| 500 mg | 5 mL | 100 mg/mL | 50 units |
| 500 mg | 10 mL | 50 mg/mL | 100 units |
Before choosing a larger volume, check that the vial has headspace for it. A 10 mL vial of bacteriostatic water reconstitutes two 500 mg vials at 5 mL each.
Reading the concentration on a U-100 syringe
A U-100 insulin syringe is graduated in units, and 100 units equal 1 mL. Units are therefore millilitres multiplied by 100: 0.1 mL is 10 units and 0.25 mL is 25 units. A 0.5 mL syringe carries 50 units and a 0.3 mL syringe carries 30 units on the same scale, with finer markings that make small volumes easier to read.
To find the volume that contains a given amount, divide the amount by the concentration. At 100 mg/mL (a 500 mg vial with 5 mL of water), the volume that contains 50 mg is 50 divided by 100, which is 0.5 mL, or 50 units. The table below runs the same calculation for four arbitrary amounts. They illustrate the arithmetic and nothing else.
| Amount (illustrative) | Volume at 100 mg/mL | U-100 units |
|---|---|---|
| 10 mg | 0.1 mL | 10 units |
| 25 mg | 0.25 mL | 25 units |
| 50 mg | 0.5 mL | 50 units |
| 100 mg | 1 mL | 100 units |
At 200 mg/mL, which the same vial gives with 2.5 mL of water, every volume in the table moves in proportion: a 50 mg draw measures 25 units instead of 50. Volumes below about 0.1 mL are hard to read accurately on a 1 mL syringe. Either move to a 0.3 mL or 0.5 mL syringe, or reconstitute with more water so that the same mass occupies a larger, more legible volume.
NAD+ is supplied by Vistara Labs as a lyophilised material for research use. The quantities on this page illustrate the arithmetic of dilution and measurement only.
The NAD+ vial
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme rather than a peptide, but it is supplied the same way: a lyophilised powder in a 500 mg vial. That is fifty times the mass of a typical 10 mg peptide vial, so it takes proportionally more water; 5 mL gives 100 mg/mL, and the vial’s headspace is the practical limit on going higher. Why NAD+ is not a peptide is covered in its own note. Certificates of analysis for the catalogue are published on the Résultats de laboratoire page. The Vaporisateur nasal NAD+ is supplied pre-mixed and needs no reconstitution; this page is for the lyophilised vial only.
At this mass the concentrations are much higher than for a peptide vial, and so are the stakes of labelling: 100 mg/mL and 50 mg/mL look alike on a handwritten label but differ twofold. NAD+ in solution is also less stable when it is kept warm, so a reconstituted vial belongs in the refrigerator between uses. NAD+ is often confused with NADH, its reduced form, and with the precursors NR and NMN; the certificate should name NAD+ itself. The human research, mostly built on oral precursors, is summarised in NAD+ restoration: what the research shows, and the NAD+ nasal spray is an option for researchers who want to skip reconstitution entirely.
Reconstitution technique
Let the vial reach room temperature, swab both stoppers, and run the measured water slowly down the inside wall of the vial rather than onto the powder. Swirl or tilt gently until the cake dissolves, never shake, and give any remaining powder a few minutes to settle into solution. Label the vial with the date and the concentration, then keep it refrigerated at 2 to 8 °C and protected from light. The full method, with the reason behind each step, is in l'achat d'eau bactériostatique au Canada.
Why the solution should look clear
Properly reconstituted NAD+ forms a clear, colourless solution. Cloudiness, strands or a gel that will not disperse usually mean the water went in too fast or the vial was shaken; haze that appears later points to warm or bright storage. The causes are covered in why peptides gel or go cloudy.
Bacteriostatic water, not sterile water
Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. The difference is explained in bacteriostatic water explained.
Questions fréquentes
How much bacteriostatic water do I add to a 500 mg vial of NAD+?
There is no single correct volume. With 5 mL of bacteriostatic water a 500 mg vial of NAD+ gives 100 mg/mL; with 2.5 mL it gives 200 mg/mL. Adding more water does not change the quantity in the vial, it spreads it through a larger volume, so write the concentration you chose on the vial.
How many 500 mg vials does a 10 mL vial of bacteriostatic water cover?
A 10 mL vial of bacteriostatic water reconstitutes two 500 mg vials at 5 mL each. Check that the peptide vial has headspace for the volume before choosing a larger one.
How many units on a U-100 syringe contain 50 mg at 100 mg/mL?
At 100 mg/mL (a 500 mg vial with 5 mL of water), the volume that contains 50 mg is 50 divided by 100, which is 0.5 mL, or 50 units. Units are millilitres multiplied by 100, so 0.1 mL is 10 units. The amounts on this page illustrate the arithmetic only.
What should reconstituted NAD+ look like?
Properly reconstituted NAD+ forms a clear, colourless solution. Cloudiness, strands or a gel that will not disperse usually mean the water went in too fast or the vial was shaken; haze that appears later points to warm or bright storage. More on the causes in why peptides gel or go cloudy.
Can I use sterile water instead of bacteriostatic water?
Only for a vial that will be accessed once. Bacteriostatic water is sterile water with 0.9% benzyl alcohol, a preservative that allows a refrigerated vial to be accessed more than once; plain sterile water is intended for single access. Details in bacteriostatic water explained.
How should a reconstituted NAD+ vial be stored?
Refrigerated at 2 to 8 °C, protected from light and labelled with the date and the concentration. Unopened lyophilised vials are kept cold and dark until they are needed. The full storage guide is how to store research peptides.
Work it out for a different vial or concentration
Our calculateur de peptides opens pre-set for a 500 mg vial with 5 mL of water and marks the 50 mg draw of 50 units on a syringe diagram. Change the vial size, water volume, amount or syringe and it recalculates.
Products: NAD+ 500 mg et bacteriostatic water 10 mL.
Références
Les travaux humains sur le NAD+ relèvent surtout de la pharmacologie et de la tolérabilité plutôt que des résultats cliniques. Une revue systématique de 2026 ne rapporte aucun essai admissible à résultats cliniques du NAD+ intraveineux ou intramusculaire lui-même pour l'anti-âge ou le mieux-être.
- NAD⁺ supplementation for anti-aging and wellness: A PRISMA-guided systematic review of preclinical and clinical evidenceRevue systématique PRISMA; 113 études d'intervention dont 33 études humaines. Ne rapporte aucun essai admissible à résultats cliniques du NAD+ intraveineux ou intramusculaire lui-même pour l'anti-âge ou le mieux-être.
- A Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NADÉtude pilote de pharmacologie humaine; métabolome NAD+ du plasma et de l'urine pendant une perfusion intraveineuse de 6 heures. Pas un essai d'efficacité.
- Intravenous infusion of nicotinamide adenine dinucleotide (NAD(+)) versus nicotinamide riboside (NR): a retrospective tolerability pilot study in a real-world settingÉtude pilote rétrospective de tolérabilité comparant quatre jours de NAD+ intraveineux à du riboside de nicotinamide intraveineux en contexte réel.
- Oral LNAD+ rapidly elevates whole blood intracellular NAD and metabolic flux without elevating plasma NAD: evidence from a randomized controlled trialEssai contrôlé randomisé d'une formulation ORALE modifiée spécifique de NAD+; résultats cliniques exploratoires et propres à la formulation.
Les liens ouvrent la fiche de l'étude sur PubMed afin que la source puisse être vérifiée directement. Citer une étude ne signifie pas que ses résultats s'appliquent à un autre usage, produit ou mode d'administration. Usage laboratoire uniquement.
